| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Enterocloster bolteae | TTGCGTTTTGGGCACAGC | TGAGGCAATACCGGTCAGC | 59.89 | 59.78 | 214 | 176 |
100.00%
|
50.00%
|
| Primer 2 | Enterocloster bolteae | TGCGGTATATGCCCTGCTG | TGCAGCCACAAATGCACC | 59.93 | 59.26 | 172 | 171 |
100.00%
|
50.00%
|
| Primer 3 | Enterocloster bolteae | TTGCGTTTTGGGCACAGC | GATTCGTGAACCGCTGTGC | 59.89 | 59.87 | 192 | 168 |
100.00%
|
50.00%
|
| Primer 4 | Enterocloster bolteae | AGCAAGCTCCAACCGCAT | GCAAGTTCTGGCGTTGGTC | 59.97 | 59.72 | 250 | 149 |
100.00%
|
50.00%
|
| Primer 5 | Enterocloster bolteae | AGCAAGCTCCAACCGCAT | TGCAAGTTCTGGCGTTGG | 59.97 | 58.57 | 251 | 149 |
100.00%
|
50.00%
|
| Primer 6 | Enterocloster bolteae | GTTTGCCGTGTCCTTCGTG | ACCTGCAAAGGCGTGTGT | 59.72 | 60.12 | 180 | 148 |
100.00%
|
50.00%
|
| Primer 7 | Enterocloster bolteae | GGTGTACCGAAGCCAGCAT | TCTGGTTGCACCGGTTGT | 60.08 | 59.41 | 182 | 148 |
100.00%
|
50.00%
|
| Primer 8 | Enterocloster bolteae | GGACAAAGCATCACGCAGC | TCTGGTTGCACCGGTTGT | 60.15 | 59.41 | 299 | 148 |
100.00%
|
50.00%
|
| Primer 9 | Enterocloster bolteae | AGCAAGCTCCAACCGCAT | TTCCTCGGACCCTTGCAAG | 59.97 | 59.63 | 264 | 140 |
100.00%
|
50.00%
|
| Primer 10 | Enterocloster bolteae | GCGATGGATGCATGAGGGA | ATCGTACACGCTGCGGAT | 59.93 | 59.19 | 182 | 140 |
100.00%
|
50.00%
|